In 2008 the first set of guidelines for standardizing research in autophagy was published. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Finally, in 2016 arose the third set of guidelines for the selection and interpretation of methods for use by investigators who aimed to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.
As wrote in the guidelines: « These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation, it is imperative to target by gene knockout or RNA interference more than one autophagy-related protein. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways implying that not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular assays, we hope to encourage technical innovation in the field. »
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COST is supported by EU Framework Programme Horizon 2020.
TRANSAUTOPHAGY COST ACTION
Neuroscience Institute, Faculty of Medicine Universitat Autónoma de Barcelona